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3D FloTrix™ MSC Chemically Defined Medium

Xeno-free, chemically-defined and exosome-free cell culture medium for expansion and large-scale production of mesenchymal stem cells and exosome collection.

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Overview

3D FloTrix™ MSC Chemically Defined Medium introduces a paradigm-shifting approach to cell cultivation with its meticulously engineered, xeno-free platform. By systematically replacing all animal-derived components—including serum and platelet lysate—with precisely formulated recombinant alternatives, this refined system delivers exceptional batch consistency and regulatory alignment. Designed to maximize MSC proliferation while safeguarding cellular integrity, it creates the optimal framework for advanced therapeutic research and industrial-scale generation of stem cells and exosomes, representing a fundamental advancement in manufacturing precision for regenerative medicine.

Applications

● Clinical-Grade Cell Therapy Manufacturing

● Industrial-Scale MSC Bioprocessing

● High-Purity Exosome Production & Research

● Regenerative Medicine & Immunotherapy R&D

Product Features:

● Complete serum-free formulation with no animal/human components using exclusively recombinant proteins.
● Enhanced cell proliferation capacity supporting rapid expansion with maintained potency and minimal senescence.
● Ready-to-use system requiring no additional coating or supplementary components for immediate implementation.
● Optimized particle performance maintaining background levels as low as ~10⁷ particles/mL for clean exosome studies.
● Regulatory-compliant platform meeting stringent quality standards with full documentation for therapeutic applications.
● Scalable production capability enabling seamless transition from research to industrial manufacturing.
● Superior batch consistency ensuring reproducible results through rigorous quality control protocols.

Results

2D Expansion: > 8-fold UCMSC expansion, comparable to hPL-supplemented SFM and superior to other commercial CDMs.

3D Expansion: Enables consistent passaging of UCMSCs on 3D TableTrix™ microcarrierswith ~ 6-fold expansion per passage.

Morphology AnalysisPhenotyping

Spindle-shaped with spiral distribution on 2D culture | Live cell staining of MSCs on 3D TableTrix™ microcarriers

Phenotyping

Typical surface markers retained
(P6 MSCs harvested from 3D TableTrix™ microcarriers)

Trilineage Differentiation

Trilineage differentiation capacity preserved
(P6 MSCs harvested from 3D TableTrix™ microcarriers)

Genetic Stability

Chromosomal karyotype stability confirmed
(P6 MSCs harvested from 3D TableTrix™ microcarriers)

coming soon

Brochure

3D FloTrix™ MSC Chemically Defined Basal Medium 2025.11.4

coming soon

Apr 11,2025

Catalogue No./ Model	Product Name	Specification
RCD231-PYJ	3D FloTrix™ MSC Chemically Defined Basal Medium	500 mL/Bottle
RCD231-B	3D FloTrix™ MSC Chemically Defined Supplement	25 mL/Bottle