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3D FloTrix™ MSC Chemically Defined Medium

Xeno-free, chemically-defined and exosome-free cell culture medium for expansion and large-scale production of mesenchymal stem cells and exosome collection.

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Overview

The 3D FloTrix™ MSC Chemically Defined Medium is a xeno-free, chemically defined platform designed to support scalable mesenchymal stem cell (MSC) culture. By replacing animal-derived components—including serum and platelet lysate—with recombinant and defined alternatives, the formulation enables improved process control, batch consistency, and regulatory alignment. The medium supports robust MSC proliferation while maintaining cell integrity and key functional characteristics, facilitating both process development and manufacturing workflows. Its defined composition enables reproducibility and scalability, supporting applications ranging from research to industrial production of MSCs and exosomes.

Applications

● Clinical-Grade Cell Therapy Manufacturing

● Industrial-Scale MSC Bioprocessing

● High-Purity Exosome Production & Research

● Regenerative Medicine & Immunotherapy R&D

Product Features:

● Completely serum-free, animal component–free formulation composed exclusively of recombinant proteins.
● Enhanced cell proliferation capacity enabling rapid expansion while maintaining potency and minimising senescence.
● Ready-to-use system requiring no additional coating or supplementary components for immediate implementation.
● Optimised particle performance, maintaining background levels as low as ~10⁷ particles/mL to support clean exosome studies.
● Regulatory-compliant platform meeting stringent quality standards with full documentation for therapeutic applications.
● Scalable production capability enabling seamless transition from research to industrial manufacturing.
● Superior batch consistency ensuring reproducible results through rigorous quality control protocols.

Results

**2D Expansion:** >8-fold UC-MSC expansion, comparable to hPL-supplemented SFM and superior to other commercial chemically defined media.

**3D Expansion:** Enables consistent passaging of UC-MSCs on 3D TableTrix™ Microcarriers, achieving ~6-fold expansion per passage.

Morphology Analysis Phenotyping

Spindle-shaped with spiral distribution on 2D culture | Live cell staining of MSCs on 3D TableTrix™ Microcarriers

Phenotyping

Typical surface markers retained
(P6 MSCs harvested from 3D TableTrix™ Microcarriers)

Trilineage Differentiation

Genetic Stability

coming soon

Brochure

3D FloTrix™ MSC Chemically Defined Basal Medium 2025.11.4

coming soon

Apr 11,2025

Catalogue No./ Model	Product Name	Specification
RCD231-PYJ	3D FloTrix MSC Chemically Defined Basal Medium	500 mL/Bottle
RCD231-B	3D FloTrix MSC Chemically Defined Supplement	25 mL/Bottle