[Introduction]

Background:

Currently, the main production methods for gene therapy projects include adherent cultivation, microcarrier cultivation, and suspended cultivation. Due to the fundamental differences in the cultivation methods of adherent and suspended cells, they exhibit variations in cell morphology, metabolism, and growth behavior, significantly impacting the quality and yield of the final products. Therefore, choosing the right cell cultivation process is of great significance for large-scale virus production.

Limitations:

While suspended cultivation is convenient and efficient, the high cost and long duration of cell suspension domestication require substantial financial and time support, making it unsuitable for early-stage cultivation such as research, small-scale trials, and pilot-scale trials. Adherent cultivation involves significant manual, space, and time costs, and the virus production efficiency is not high. Therefore, microcarrier cultivation is an excellent choice for the research, small-scale trials, and pilot-scale trials stages, providing a new option, especially for 293 and Vero cells that cannot undergo suspended cultivation.

 Breakthrough:

In response to industry challenges, Cytoniche has developed the corresponding 3D FloTrix® large-scale cell cultivation process — the 3D TableTrix® G Series three-dimensional porous degradable microcarriers. Combined with a bioreactor, it achieves suspended cultivation of adherent cells. Subsequently, through incremental amplification, large-scale, high-quality cell amplification can be realized.

[3D TableTrix® G Series Microcarriers]

▣ Sterile Packaging

No preprocessing is required, can be directly connected to a bioreactor for closed cultivation.

▣ 3D Biomimetic Elastic Porous Structure

Pore size range of 30-50μm, porosity >90%, providing a biomimetic growth environment for cells, ensuring stronger adhesion to the microcarriers.

Effectively reduces shear forces caused by stirred reactors, protecting host cells and reducing host cell residue.

▣ Surface Area up to 9000cm²/g

Provides ample growth space for cells.

▣ Mild and Non-Destructive Degradation, High Cell Recovery Rate

Suitable for continuous amplification cultivation.

 ▣ Applicable to Various Adherent Cell Lines such as 293T, Vero

Widely used in large-scale production of lentivirus, adenovirus, AAV virus, oncolytic virus, and more.

[3D FloTrix® Large-Scale Cell Cultivation Technology for Virus Production Processes]

Compared to traditional carriers, the 3D TableTrix® G Series Microcarriers show significant advantages in cell amplification and virus production.

 Used for large-scale cultivation of 293T cells, cell density can reach 1 × 10⁷ cells/mL. Cells can be steadily amplified in a bioreactor, ultimately achieving high-titer production of secreted and intracellular viruses.

Efficient and high-yield application processes will be shared soon!

Stay tuned for more updates.

【CytoNiche】

CytoNiche was established in 2018, led by Professor Yanan Du's research team from the School of Medicine at Tsinghua University, with Tsinghua University as a shareholder. The core technology originated from the transformation of scientific achievements at Tsinghua University and was recognized as a leading technology in "Science and Technology Innovation in China" by the China Association for Science and Technology. As a national-level high-tech enterprise, a national-level specialized and new technology "Little Giant" enterprise, a potential unicorn enterprise, it has also received key research and development special support from the Ministry of Science and Technology.
As an expert in high-quality three-dimensional cell manufacturing, CytoNiche provides a one-stop customized solution for cell scale-up based on 3D microcarriers. The company has built an original 3D cell smart manufacturing platform, achieving large-scale, automated, intelligent, and closed-cell drug and derivative production preparation. This helps global customers establish the most advanced cell drug production lines. After pioneering the production process pipeline for "billion-level" stem cells, the company is accelerating towards "hundred billion-level," dedicating efforts to empower the cell and gene therapy industry with 3D cell scale-up smart manufacturing technology to benefit more patients.